Table 1.
Microarray genes down-regulated by dietary cholesterol
| Mean fold decrease | GenBank accession no. | Gene product | Fold decrease on six arrays
|
|||||
|---|---|---|---|---|---|---|---|---|
| 1a | 1b | 2a | 2b | 3a | 3b | |||
| 4.6 | AA237469 | Isopentenyl diphosphate isomerase | 3.3 | 7.6 | 2.4 | 3.6 | 4.9 | 5.9 |
| 2.8 | AA239481 | Uncharacterized EST (StarD4) | 3.6 | 2.3 | 4.8 | 1.6 | 2.8 | 1.9 |
| 2.6 | AA268608 | Squalene epoxidase | 3.2 | 2.7 | 2.8 | 1.3 | 2.9 | (44) |
| 2.5 | AA061468 | Hydroxymethylglutaryl-CoA synthase | 2.6 | 0.9 | 5.2 | 2.0 | 2.4 | 2.1 |
| 2.0 | AA116513 | Fatty acid synthase | 1.4 | 1.7 | 2.0 | 2.8 | 2.3 | 2.0 |
| 2.0 | AA500330 | Farnesyl pyrophosphate synthetase | 1.2 | 1.7 | 2.0 | 2.8 | 2.3 | 2.0 |
Experiments 1–3 each compared liver gene expression in a pair of individual mice fed different diets (0.02% versus 0.5% cholesterol). Each experiment was performed on duplicate arrays (a, b) by reversing the Cy3 and Cy5 probe labeling. Since there was marked variability within and between experiments, the following criteria were used for regulated genes: expression differed 2-fold or greater on at least four of the six arrays with the higher expression level at least 25% over background. For each of the six genes down-regulated by the high-cholesterol diet, the fold regulation on each array is shown, as well the average from all six (the outlying value in parenthesis was eliminated).