Table 10.
Effect of water activity on the viability of B. subtilis HU58 spores in cookies.
| Time (months) | Freezer temperature (−18°C) | Refrigerator temperature (4°C) | Room temperature (25°C) |
|---|---|---|---|
| 1 | 0.54 ± 0.0 A | 0.63 ± 0.1 A | 0.53 ± 0.0 A |
| 2 | 0.54 ± 0.0 A | 0.55 ± 0.1 A | 0.59 ± 0.1 A |
| 4 | 0.57 ± 0.0 A | 0.49 ± 0.0 B | 0.57 ± 0.0 A |
| 6 | 0.55 ± 0.0 A | 0.46 ± 0.1 A | 0.58 ± 0.0 A |
| 8 | 0.62 ± 0.0 A | 0.44 ± 0.1 A | 0.49 ± 0.1 A |
| 10 | 0.58 ± 0.0 A | 0.36 ± 0.1 B | 0.34 ± 0.0 B |
| 12 | 0.56 ± 0.0 A | 0.31 ± 0.0 B | 0.37 ± 0.0 C |
Note: Different capital letters (A) in the same row denote significant differences (p ≤ 0.05) in log reductions between the different holding temperatures.