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. 2002 May 7;99(10):7009–7014. doi: 10.1073/pnas.092562399

Figure 2.

Figure 2

Assessment of binding of Flk-1 to cTcR-expressing cells. (A) FACS analysis demonstrating the binding of soluble Flk-Fc only to HeLa cells expressing VEGF-cTcR. CM, conditional medium. (B) Binding of soluble human KDR-Fc to a CTL clone expressing VEGF-cTcR (VEGF-cTcR clone 2). Cells were incubated with increasing concentrations of soluble KDR-Fc, washed, incubated with anti-human IgG-FITC, and subjected to FACS analysis. The data are plotted on a logarithmic scale. (Insert) Plot of the data on a linear scale to demonstrate saturable binding.