Figure 3.

Down-regulation of SNS marker genes and induced expression of neural crest genes in hypoxic NB cells. (A) Western blot analysis of chromogranin and TH in NB cells exposed to 21% or 1% oxygen for 72 h. (B and C) Northern blot analysis of neuronal and neural crest marker genes in normoxic and hypoxic NB cells grown for 4 (B) and 72 h (B and C). We used 28S-RNA as a loading control. (D) Western blot analysis of Notch-1 in cells exposed to normoxia or hypoxia for 72 h. (E) Reverse transcription–PCR analysis of c-kit expression in hypoxic (72 h) NB cells. Beta-2-microglobulin (β₂M) was a reference for input cDNA. Representative data of more than two experiments are shown.