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. 2025 Sep 19;14(3):74. doi: 10.3390/biotech14030074

Table 2.

Representative fermentation conditions and reported volumetric activities for bacterial and fungal pectinase producers.

Microorganism Substrate Fermentation Pectinase Activity Ref.
Type Time T °C pH Agitation
B. amyloliquefaciens TKU050 Wheat bran SSF 4 days 37 6.0 100 rpm 0.76 U/mL [48]
B. amyloliquefaciens SL9 Pectin SmF 24 h 37 7.0 150 rpm 9.8 U/mL [23]
B. subtilis NRRL B-4219 Hazelnut shell
hydrolyzate
SmF 72 h 30 7.0 130 rpm 5.60 U/mL [52]
B. pumilus NRRL B-212 Pectin SmF 64 h 30 8.0 150 rpm 16.17 U/mL [49]
Sugar beet pulp SSF 48 h 30 8.0 150 rpm 147.75 U/mL
B. mojavensis I4 Carrot peels SSF 32 h 37.5 8.0 150 rpm 64.8 U/mL [53]
B. tequilensis CAS-MEI-2-33 Pectin SmF 40 h 40 10.0 180 rpm 1370 U/mL [54]
B. tropicus MCCC1A01406 Pectin SmF 72 h 37 9.0 - 43 U/mL [55]
B. amyloliquefaciens ADI2 Banana peel SSF 48 h 28 8.38 94 rpm 2043.86 U/mL [46]
B. subtilis strain Btk 27 Apple pectin SmF 48 h 37 6.5 120 rpm 66.3 U/mL [43]
B. subtilis MF447840.1 Pectin SmF 4 days 37 7.4 120 rpm 345 ± 12.3 (U/mL) [56]
B. subtilis PSE-8 Cassava peel SSF 3 days 45 9 100 rpm 117.5 (U/mL) [57]
B. cereus Pectin SmF 24 h 35 10.5 150 rpm 3.37 (U/mL) [58]
B. licheniformis Orange peel SSF 120 h 37 9.5 - 219 (U/mL) [59]
B. subtilis ZGL14 Pectin SmF 72 h 40 8.0 200 rpm 734.11 (U/mL) [60]
Bacillus sp. Y1 Wheat bran SmF 72 h 37 8.2 100 rpm 40
(U/mL)
[61]
B. safensis M35 Citrus peel & Wheat bran SSF 72 h 37 5.8 160 rpm 411.58 (U/mL) [62]
B. altitudinis J208 Citrus peel & Wheat bran SSF 72 h 37 6.2 160 rpm 728.74 (U/mL) [62]
Aspergillus niger Apple pomace SSF 96 h 25 4.0 - 6.75 U/mL [63]
Aspergillus aculeatus NEJC Mango peel SSF 8 days 40 5.5 - 1360 U/mL [64]
Aspergillus niger AUMC16245 Pectin SmF 7 days 40 7.0 200 rpm 3787.04 U/mL [65]
Aspergillus brasiliensis AUMC16244 Pectin SmF 5 days 45 7.0 200 rpm 3878.35 U/mL [65]
Aspergillus niveus AUMC1624 Pectin SmF 7 days 45 7.0 200 rpm 3572.95 U/mL [65]
Aspergillus foetidus Mango peel SSF 96 h 30 5.5 - 228 U/mL [66]
Aspergillus spp. Gm 0.5% Pectin SmF 48 h 30 5.8 150 rpm 112 U/mL [44]
Saccharomyces cerevisiae Corn and orange peels SSF 6 days 30 4.0 - 29.57 U/mL [67]
Streptomyces halstedii Citrus pectin SmF 24 h 28 8.0 200 rpm 1.052 U/mL [68]

B.: Bacillus, Enzyme activity (U/mL): Volumetric activity, units per milliliter of fermentation broth.