Figure 4.
Immunoprecipitation analysis of DEFs uninfected (lane 1) and infected with recombinant rMd5 (lane 2) and pp38 deletion mutant rMd5Δpp38-19 (lane 3). Immunoprecipitation of [35S]methionine-labeled cells was done with mAbs specific to pp38 (H19; Top) or pp40 (2BN90; Bottom). H19 not only immunoprecipitates pp38 but also coprecipitates pp24 in rMd5-infected cells. As expected, in the absence of pp38 (rMd5Δpp38), pp24 is not precipitated by H19. On the other hand, 2BN90 immunoprecipitates pp40 in both virus lysates.