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Production of exotoxin A in response to the signaling pathway. Culture supernatants for the strains shown, which had been grown with or without the addition of pyoverdine, were serially diluted. and the presence of exotoxin A was determined with an anti-exotoxin antibody. toxA promoter activity was determined by measuring the amounts of β-galactosidase produced by strains carrying the toxA-lacZ reporter fusion plasmid construct pPZ-toxA. All SDs were less than 12%. ND, not determined.
