Table 2.
Growth and pyoverdine production of strains of P. aeruginosa
| Strain | Sensitivity to EDDA*
|
Pyoverdine, μM† | |
|---|---|---|---|
| +EDDA | −EDDA | ||
| PAO | +++ | +++ | 103.4 (12.1) |
| PAOfpvR | +++ | +++ | 81.9 (8.0) |
| PAO1FpvR++ | − | +++ | <1.0 |
| PAO1pvdS | − | +++ | <1.0 |
| PAO1pvdF | − | +++ | <1.0 |
| PAO1fpvA | − | +++ | 31.7 (3.1) |
| PAO1fpvAfpvR | + | +++ | 85.9 (5.6) |
*
Bacterial strains were grown on King's B agar with (+EDDA) or without (−EDDA) the iron-chelating compound EDDA. +++, wild-type levels of growth; +, slight growth; −, no growth; EDDA, ethylenediamine-di(O-hydroxy-phenylacetic acid).
†
The amounts of pyoverdine present in the supernatants of cultures grown without EDDA were determined as described in Materials and Methods. Values shown are the averages of at least three experiments with SDs shown in parentheses.