Figure 2.
Redox titration of disulfide bond formation in CrtJ. The presence of reactive thiols was measured by analysis of the fluorescence emission level of CrtJ after incubation with mBBr at various redox values under anaerobic (argon) conditions. The filled circles represent the fluorescence emission at pH 8.0 and the open circles is at pH 7.0. The amplitudes are on a scale where the amplitude measured at the most negative redox value is a value of 1.0. The line represents a fit of the data to a two-electron Nernst curve. Eh (mV) denotes actual potentials.