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. 2002 Apr 30;99(10):7090–7095. doi: 10.1073/pnas.092013799

Figure 5.

Figure 5

Defects in the secondary proliferative cell population that forms the DG. (A–D) Coronal sections through the E18.5 hippocampus of wild-type (A and C) or CXCR4 mutant mice (B and D), processed to show expression of Prox1 (blue) or Prox1 together with BrdUrd-labeled dividing cells (orange). In the wild-type mouse, Prox1 is expressed in the forming dg (A and C). By contrast, in the mutant, Prox1 is expressed in the vestigial dg but also along the migratory stream (ms) (arrows in D) of dividing cells running along the ventral surface of the hippocampus into the dg. BrdUrd-labeled cells of the ms can be seen between the two arrows in C. (E) A higher magnification of the migrating stream of cells shown in D. Numerous blue, Prox1-expressing cells appear among the brown, BrdUrd-labeled cells, but the populations appear largely distinct. Arrows in E point to two single-labeled cells. (F and G) High-magnification views of BrdUrd-labeled dividing cells (dark blue) coursing through the ms in a wild-type (F) and a CXCR4 mutant mouse (G). About 30% fewer BrdUrd-labeled cells appear in the ms of the mutant (G) than in the wild type (F).