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. 2002 May 14;99(10):7096–7101. doi: 10.1073/pnas.102156099

Figure 2.

Figure 2

Surface expression of FLAG-GFP-GluR2 mutant constructs in cultured hippocampal neurons. (a–d) Examples of hippocampal neurons transfected with FLAG-GFP-GluR2 mutant constructs (green) all showing surface expressed receptors (red). (Insets) Higher power view of surface expressed puncta of FLAG-GFP-GluR2 mutant constructs. (a) FLAG-GFP-GluR2(ΔNSF). (b) FLAG-GFP-GluR2(S880A). (c) FLAG-GFP-GluR2(Del3aa). (d) FLAG-GFP-GluR2(S880D). (e) Quantification of the proportion of AMPARs on the cell surface compared with the total shows no difference between constructs (ANOVA: df = 4, MS = 79.5, F = 1.07, P = 0.37).