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. 2002 May 14;99(10):7096–7101. doi: 10.1073/pnas.102156099

Figure 3.

Figure 3

Constitutive endocytosis of FLAG-GFP-GluR2 is not influenced by binding to cytoplasmic proteins. (a–c) Representative images of transfected cells in which constitutive endocytosis of surface-expressed AMPARs was imaged. (Left) GFP fluorescence (green). (Center) Receptors that were surface labeled and then constitutively internalized (red). (Right) Remaining surface receptors (blue). (a) FLAG-GFP-GluR2. (b) FLAG-GFP-GluR2(ΔNSF). (c) FLAG-GFP-GluR2(S880A). (d) Quantification of the proportion of the total amount of surface-expressed AMPARs that were internalized shows no significant difference between constructs (ANOVA: df = 4, MS = 12.7, F = 0.60, P = 0.67).