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. 2002 May 14;99(10):7096–7101. doi: 10.1073/pnas.102156099

Figure 5.

Figure 5

AMPA-induced endocytosis of FLAG-GFP-GluR2 is regulated by interaction with cytoplasmic proteins. (a–c) Representative images of transfected cells in which AMPA-induced endocytosis of FLAG-GFP-GluR2 constructs was imaged. Panels are the same as in Fig. 3. (a) FLAG-GFP-GluR2. (b) FLAG-GFP-GluR2(ΔNSF). (c) FLAG-GFP-GluR2(S880A). (d) Quantification shows all constructs exhibit significant internalization (P < 0.005) on AMPA stimulation (filled columns) compared with control conditions (open columns). There is significantly increased AMPA-induced internalization of FLAG-GFP-GluR2(ΔNSF) (*, P < 0.05) and decreased internalization of FLAG-GFP-GluR2(S880A), FLAG-GFP-GluR2(S880D), and FLAG-GFP-GluR2(Del3aa) compared with FLAG-GFP-GluR2 (*, P < 0.05). There is no significant difference between constructs in the absence of AMPA (open columns). ANOVA: df = 4, MS = 62.4, F = 0.98, P = 0.42.