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. 2002 May 14;99(10):7096–7101. doi: 10.1073/pnas.102156099

Figure 6.

Figure 6

The intracellular pool of internalized FLAG-GFP-GluR2 unable to interact with GRIP/ABP is dissipated 1 h after stimulation. One hour after AMPA treatment, FLAG-GFP-GluR2 and FLAG-GFP-GluR2(ΔNSF) still show a significant increase in the amount of internalized receptors compared with untreated cells (P < 0.05) whereas the mutants unable to interact with GRIP/ABP or PICK1 do not [FLAG-GFP-GluR2(S880A), P = 0.57; FLAG-GFP-GluR2(S880D), P = 0.25; FLAG-GFP-GluR2(Del3aa), P = 0.60). There is no significant difference in magnitude of AMPAR-induced internalization of FLAG-GFP-GluR2(ΔNSF) (P = 0.64), but decreased internalization of FLAG-GFP-GluR2(S880A), FLAG-GFP-GluR2(S880D), and FLAG-GFP-GluR2(Del3aa) compared with FLAG-GFP-GluR2 (*, P < 0.05). There is no significant difference between constructs in the absence of AMPA (open bars). ANOVA: df = 4, MS = 30.4, F = 0.61, P = 0.65).