Figure 1.
(A) Effects of daily s.c. injections of saline (n = 7 for each control group) or human GH (hGH) (1 mg/kg, n = 7 for each hGH-treated group) on NMDA receptor subunit, GHR and GHBP mRNAs in the adult rat hippocampus. *, P < 0 05; **, P <0.01; ***, P < 0.001 compared with the age-corresponding control group; †, P < 0.05, ‡‡, P < 0.01 compared with the 11-week-old control animals (unpaired Student's t test). (B) Representative Northern blot analysis of NMDA subunits, GHR, and GHBP mRNAs. Each well was loaded with 20 μg of total RNA. For correction of interlane variability in the amount of total RNA, the hybridization signals were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA.