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. 2002 May 14;99(10):7166–7171. doi: 10.1073/pnas.062053099

Figure 1.

Figure 1

Purification of recombinant R1. Proteins were separated by SDS/PAGE and stained with Coomassie (lanes M and 1–3) or subsequently transferred to nitrocellulose and probed with an antibody raised against R1 (lane 4). M, molecular weight marker; 1, crude E. coli extract following induction (10 μg); 2, protein fraction derived from anion-exchange chromatography (10 μg); 3, purified R1 following passage through the starch column (2.5 μg); 4, Western blot of the purified R1-fraction (0.7 μg).