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G6P and G3P residues are products of in vitro phosphorylation by R1. Following the coelongation assay (7.2 μg R1,5 mM ATP, 14 h incubation) the synthesized polyglucan was precipitated and hydrolyzed. Samples containing equal amounts of glucose were then analyzed by HPAEC-PAD. The elution profile of authentic G6P and G3P (4 nmol each) is also shown.
