Table 3.

List of clinical strains used for primers and probe validation.

ARG variants tested	Reference strain	Internal reference*	Expected	Experimental validation (Mean Ct of 4 replicates)
aadA1	Acinetobacter baumannii	5f5a9457	+	+ (19.97)
aadA1	Acinetobacter baumannii	e70321e7	–	–
aadA2	Enterobacter cloacae	743a5edb	+	+ (24.72)
aadA2	Enterobacter cloacae	c6ec50ff	–	–
aph(3′')-Ib	Pseudomonas aeruginosa	9afcc077	+	+ (18.03)
blaTEM-1	Escherichia coli	0259bf71	+	+ (18.40)
blaTEM-1	E. coli	6961107c	–	–
blaTEM-12	Acinetobacter baumannii	aa4e92ba	+	+ (20.76)
blaTEM-12	Acinetobacter baumannii	e70321e7	–	–
blaTEM-135	Klebsiella pneumoniae	b7f02bd9	+	+ (17.48)
blaTEM-135	Acinetobacter baumannii	e70321e7	–	–
blaTEM-150	Klebsiella pneumoniae	409df3b6	+	+ (19.68)
blaTEM-150	Klebsiella pneumoniae	600819d2	–	–
blaTEM-35	E. coli	569ba05d	+	+ (19.01)
blaTEM-35	E. coli	6961107c	–	–
ermF	Acinetobacter baumannii	aa4e92ba	–	–
mcr-1.8	E. coli	dbde13a4	+	+ (17.45)
mcr-1.8	E. coli	0259bf71	–	–
mecA	Staphylococcus aureus	00e009e5	+	+ (18.01)
mecA	E. coli	7d243655	–	–
qnrS1	E. coli	01eb6b0b	+	+ (20.26)
qnrS1	E. coli	0259bf71	–	–
qnrS13	E. coli	6961107c	+	+ (24.30)
qnrS13	E. coli	0259bf71	–	–
tetA	Enterobacter cloacae	743a5edb	+	+ (20.03)
tetA	Enterobacter cloacae	c6ec50ff	–	–
tetB	E. coli	1b764e79	+	+ (18.30)
tetB	E. coli	0259bf71	–	–

^⁎The internal reference code of Applied Microbiology Research Lab, Institute of Medical Microbiology, University of Zurich.