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. 2025 Sep 10;20(9):2308–2317. doi: 10.1021/acschembio.5c00475

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Cofactor profiling of the NR2F6 LBD. (A) Domain organization of NR2F6 and concept of LBD-mediated recruitment of coregulator proteins. (B) TR-FRET cofactor profiling of NR2F6 LBD. Data recorded in triplicate. (C) Sequence alignment of screened noncanonical NR coregulator peptides. (D) TR-FRET concentration–response curve of bio-NSD1 binding to His-NR2F6 LBD. Data shown is the average and standard deviation of three independent experiments. (E) TR-FRET concentration–response curves of acetylated NSD1, BCL11A-1, BCL11A-2, FOG2 and dATRO in a bio-NSD1 displacement assay. Data were recorded in triplicate; data shown is representative of three independent experiments. Reported values represent the average and standard deviation of three independent experiments. (F) DSF curves of apo NR2F6 LBD in the presence of DMSO or NSD1, BCL11A-1, BCL11A-2, FOG2 or dATRO peptide. Data shown is the average and standard deviation of three independent experiments.