Figure 2.
The dynamics of mRNA translational fate during mouse pre-implantation development. (A, B) Fuzzy c-means clustering identified 12 distinct temporal patterns of HF-bound mRNA (A) and LF-bound mRNA (B). The ‘x’ axis represents developmental stages in time course, while the ‘y’ axis represents log2-transformed, normalized intensity ratios in each stage. (C) The heatmap showing the number of genes overlapped from the identified stage-specific clusters between heavy- and light-fraction layers based on Fig. 2A and B. The color scheme, from white to green, indicates the number of intersection genes from low to high. The genes intersected between HF peaking at two-cell stage (H_Cluster7) and LF peaking at zygote (L_Cluster3)/two-cell (L_Cluster5)/four-cell (L_Cluster7) were highlighted and further explored. (D) Heatmap showing the expression levels of representative genes (Rep. genes) across the HF, LF, and transcriptome profiles. (E) An illustration graph summarizing three different mRNA fates during early embryo development. Example given here is focusing on two-cell stage.