1. Most Abundant Proteins from E. coli in Purified Hbs Produced by the Cell-Free System Found in LC–MS.
| protein ID | accession | –10 lg P | coverage (%) | peptides number | unique peptides | description |
|---|---|---|---|---|---|---|
| 1061 | sp|B7L4K9|RL3_ECO55 | 463.42 | 72 | 19 | 19 | large ribosomal subunit protein uL3 |
| 55 | sp|A7ZHA4|DNAK_ECO24 | 583.99 | 71 | 52 | 51 | chaperone protein DnaK |
| 21 | sp|P00573|RPOL_BPT7 | 704.22 | 75 | 82 | 82 | T7 RNA polymerase |
| 4 | sp|P00490|PHSM_ECOLI | 635.42 | 81 | 70 | 70 | maltodextrin phosphorylase |
| 52 | sp|Q8XEG2|GLMS_ECO57 | 665.46 | 72 | 44 | 44 | glutamine--fructose-6-phosphate aminotransferase |
| 5 | sp|P0A6N2|EFTU_ECOL6 | 563.88 | 81 | 31 | 31 | elongation factor Tu |
| 1014 | sp|A7ZUD3|TPIS_ECO24 | 474.13 | 78 | 21 | 21 | triosephosphate isomerase |
| 852 | sp|B1IQH2|RS2_ECOLC | 496.26 | 85 | 25 | 25 | |
| 96 | sp|P35340|AHPF_ECOLI | 511.53 | 60 | 29 | 29 | alkyl hydroperoxide reductase subunit F |
a
Protein ID from the UniProt database.
b
Unique identifier assigned to each protein sequence entry in the UniProt database.
c
Protein reconstruction score by Peaks software, used in LC/MS analysis.
d
Percentage of a protein’s amino acid sequence identified by the peptides detected by LC/MS.
e
Number of detected peptides identified by spectrophotometer and used to identify the protein.
f
Number of unique peptides identified from the specific protein.