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. 2025 Aug 18;99(9):e01130-25. doi: 10.1128/jvi.01130-25

Fig 2.

Genomic diagram contains gene organization with ORFs, line graph compares nucleotide similarity and read coverage across strains by genome position, and RNA structure with stem-loop and calculated free energy value.

Molecular features of PoBastV AUS/2015 and CHE/2022. (A) Schematic representation of the genome organization. The range of the ORFs is indicated with their nucleotide position in the PoBastV-CH22 genome. Note that PoBastV AUS/2015 reveals the same genomic organization. Analysis with motif finder tool (https://www.genome.jp/tools/motif/; accessed 26 June 2023) revealed a viral methyltransferase, viral helicase 1, and RdRp2 domain in the ORF1, a capsid domain in ORF2, and a RHIM motif (RIP homotypic interaction motif) in ORF4, which has a function in regulation of apoptosis and immune modulation of cells (15, 16). The protein putatively encoded by ORFs 3A and 3B revealed no known protein motifs. Putative ORFs are indicated by question marks. (B) Pairwise comparison of nucleic acid sequence similarities (left y-axis) of PoBastV CHE/2022 and AUS/2015 (continuous lines) to the porcine bastrovirus reference strain (PoBastV Viet Nam/17489_85/2015; Genbank NC_032423) and of PoBastV AUS/2015 (dashed line) to CHE/2022. The gray shaded graph represents the coverage of the PoBastV CHE/2022 sequence by HTS reads (Integrative Genomics Viewer version 2.14.1). (C) Secondary structure prediction for the genomic 3′ UTR reveals a prominent stem-loop. Genome position of the nucleotides and the Gibbs free energy change (ΔG) are indicated.