FIG. 1.
Infection with SFFV alters the electrophoretic mobility of Raf-1 in HCD-57 cells. Raf-1 was immunoprecipitated from uninfected (lanes 1 and 5), SFFVP-infected (lanes 2 and 4), or SFFVA-infected (lanes 6 and 8) HCD-57 cells after starvation in 1.5% FCS overnight or after incubation of the starved cells for 15 min in complete medium with (lanes 3 and 7) or without (lanes 4 and 8) Epo. The immunoprecipitated proteins were resolved by electrophoresis on an SDS–7.5% polyacrylamide gel and detected by Western blotting using an anti-Raf-1 monoclonal antibody.