Table 1.

Comparison of the identified TflABH, MthABH, and OspAmd urethanases with the benchmark enzymes UMG‐SP‐1 and UMG‐SP‐2 with respect to specific activities, melting and optimal temperatures, and pH optima. All values were determined with 7‐carbethoxy‐4‐methylcoumarin (EMACC) in triplicates.

Enzyme	Specific Activity [μmol min−1 mg−1]	Optimal Temperature [°C]	Optimal pH	Melting Temperature [°C]
TflABH	0.013 ± 0.0018	60.0	8.0	70.0 ± 0.0
MthABH	0.0007 ± 0.00001	60.0	7.0	56.6 ± 0.0
OspAmd	0.292 ± 0.015	55.5	9.5	57.9 ± 0.1
UMG‐SP‐1	0.199 ± 0.026	70a)	10a)	54.6 ± 0.0
UMG‐SP‐2	0.147 ± 0.021	70a)	10a)	47.2 ± 0.0

^a)Reported by Branson et al.^{[ 33 ]}