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Reactivity of the anti-HPV VLP sera against HPV-16 L1 VLPs. ELISA plates were coated with HPV-16 VLPs and incubated with threefold serial dilutions of antisera to the following VLP types: HPV-11 (•), HPV-16 (▪), HPV-18 (▴), HPV-31 (▾), HPV-33 (○), HPV-35 (□), HPV-39 (▵), and HPV-45 (▿). Specific binding was detected with an anti-rabbit immunoglobulin G secondary antibody reagent as described in Materials and Methods. O.D., optical density.
