FIG. 4.

VZV protein synthesis in skin implants. Implants were inoculated with VZV and harvested on day 21 postinfection. Protein was extracted from infected implants, separated by SDS-PAGE, and transferred to nylon membranes. Western blot analysis was performed; VZV proteins were detected with a high-titer human polyclonal serum and ECL on a phosphorimager. The concentration of VZV protein in the range of 70 to 120 kDa was measured digitally in density units. The means and standard error are shown for each VZV strain. All paired VZV strains are statistically different from each other (P ≤ 0.04, Student’s t test) except (i) VZV-S and P-Oka and (ii) VZV-Ellen and gC⁻-Ellen.