Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| CCRF-CEM | Megrelis et al (2018) Frontiers in Immunology | Jérôme Delon |
| Recombinant DNA | ||
| LifeAct-mCherry | Molla-Herman A et al (2010) (Journal of Cell Science) | Dr A. Benmerah (Institut Imagine, France) |
| GFP-humanCentrin1 | Azimzadeh et al (2009) (Journal of Cell Biology) | Dr A. Delouvée (Institut Curie, Paris, France) |
| Centrin1-VCA-GFP and Centrin1-GFP | Obino et al (2016) (Nature Communications) | Dr P. Pierobon (Institut Cochin, Paris, France) |
| TEpacVV (H187) | Klarenbeek J et al (2015) (PLoS ONE) | K. Jalink (Netherlands Cancer Institute) |
| ExRaiAKAR2 | Addgene | Cat #161753 |
| Antibodies | ||
| Mouse anti-pericentrin | Abcam | Cat #28144 |
| Rabbit anti-pericentrin | Abcam | Cat #4448 |
| Rabbit anti-Arp2 | Abcam | Cat #47654 |
| Mouse anti-AKAP450 | Becton Dickinson | Cat #611518 |
| Mouse anti-CXCR4 human | R&D Systems | Cat #MAB172 |
| Mouse anti-α tubulin | Sigma | Cat #T9026 |
| Mouse anti-PKA Cα | Becton Dickinson | Cat #610981 |
| AlexaFluor568 donkey anti-mouse | Life Technologies | Cat #A10037 |
| AlexaFluor647 donkey anti-rabbit | Life Technologies | Cat #A32787 |
| AlexaFluor plus 647 donkey anti-mouse | Life Technologies | Cat #A31573 |
| Chemicals, enzymes, and other reagents | ||
| RPMI 1640 GlutaMax | Gibco | Cat #61870010 |
| Fetal Calf Serum (FCS) | GIBCO | Cat #A5256801 |
| Penicillin and streptomycin | Eurobio | Cat #CABPES01-OU |
| Amaxa Nucleofactor | Lonza | Cat #VCA-1003 |
| Human AB serum | Biowest | Cat #S4190–100 |
| Pan T cell Isolation Kit | Miltenyi | Cat #130-096-535 |
| recombinant human SDF1-α | Peprotech | Cat #300-28 A |
| VCAM-1 (CD106 Fc chimera protein) | R&D Systems | Cat #862-VC-100 |
| Fura-2/AM | Molecular Probes | Cat #F1225 |
| BAPTA-AM | Thermo Fisher | Cat #B6769 |
| Hoechst 33342 | Molecular Probes | Cat #H1399 |
| AlexaFluor488 conjugated phalloidin | Life Technologies | Cat #A12379 |
| AlexaFluor568 conjugated phalloidin | Life Technologies | Cat #A12380 |
| SiRActin | TebuBio | Cat #SC001 |
| H89 | Calbiochem | Cat #371963 |
| Rp-8-CPT-cAMPS | Biolog | Cat #C011 |
| Ht31 (RRRRRRRRRLIEEAASRIVDAVIEQV) | ProteoGenix | |
| Ht31-P (RRRRRRRRRLIPEAASRPVDAVIEQV) | ProteoGenix | |
| Fluorsave | Millipore | Cat #345789 |
| Transwell | Corning | Cat #3421 |
| Software | ||
| Metafluor | Meta Imaging (Roper Scientific) | |
| Metamorph | Meta Imaging (Roper Scientific) | |
| ImageJ / Fiji | https://imagej.net/ | |
| Jamovi software | https://www.jamovi.org/ | |
| Prism | GraphPad | |