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. 2025 Aug 26;26(18):4436–4455. doi: 10.1038/s44319-025-00533-2

Reagents and tools table

Reagent/resource Reference or source Identifier or catalog number
Experimental models
CCRF-CEM Megrelis et al (2018) Frontiers in Immunology Jérôme Delon
Recombinant DNA
LifeAct-mCherry Molla-Herman A et al (2010) (Journal of Cell Science) Dr A. Benmerah (Institut Imagine, France)
GFP-humanCentrin1 Azimzadeh et al (2009) (Journal of Cell Biology) Dr A. Delouvée (Institut Curie, Paris, France)
Centrin1-VCA-GFP and Centrin1-GFP Obino et al (2016) (Nature Communications) Dr P. Pierobon (Institut Cochin, Paris, France)
TEpacVV (H187) Klarenbeek J et al (2015) (PLoS ONE) K. Jalink (Netherlands Cancer Institute)
ExRaiAKAR2 Addgene Cat #161753
Antibodies
Mouse anti-pericentrin Abcam Cat #28144
Rabbit anti-pericentrin Abcam Cat #4448
Rabbit anti-Arp2 Abcam Cat #47654
Mouse anti-AKAP450 Becton Dickinson Cat #611518
Mouse anti-CXCR4 human R&D Systems Cat #MAB172
Mouse anti-α tubulin Sigma Cat #T9026
Mouse anti-PKA Cα Becton Dickinson Cat #610981
AlexaFluor568 donkey anti-mouse Life Technologies Cat #A10037
AlexaFluor647 donkey anti-rabbit Life Technologies Cat #A32787
AlexaFluor plus 647 donkey anti-mouse Life Technologies Cat #A31573
Chemicals, enzymes, and other reagents
RPMI 1640 GlutaMax Gibco Cat #61870010
Fetal Calf Serum (FCS) GIBCO Cat #A5256801
Penicillin and streptomycin Eurobio Cat #CABPES01-OU
Amaxa Nucleofactor Lonza Cat #VCA-1003
Human AB serum Biowest Cat #S4190–100
Pan T cell Isolation Kit Miltenyi Cat #130-096-535
recombinant human SDF1-α Peprotech Cat #300-28 A
VCAM-1 (CD106 Fc chimera protein) R&D Systems Cat #862-VC-100
Fura-2/AM Molecular Probes Cat #F1225
BAPTA-AM Thermo Fisher Cat #B6769
Hoechst 33342 Molecular Probes Cat #H1399
AlexaFluor488 conjugated phalloidin Life Technologies Cat #A12379
AlexaFluor568 conjugated phalloidin Life Technologies Cat #A12380
SiRActin TebuBio Cat #SC001
H89 Calbiochem Cat #371963
Rp-8-CPT-cAMPS Biolog Cat #C011
Ht31 (RRRRRRRRRLIEEAASRIVDAVIEQV) ProteoGenix
Ht31-P (RRRRRRRRRLIPEAASRPVDAVIEQV) ProteoGenix
Fluorsave Millipore Cat #345789
Transwell Corning Cat #3421
Software
Metafluor Meta Imaging (Roper Scientific)
Metamorph Meta Imaging (Roper Scientific)
ImageJ / Fiji https://imagej.net/
Jamovi software https://www.jamovi.org/
Prism GraphPad