Reagents and tools table
Reagent/resource | Reference or source | Identifier or catalog number |
---|---|---|
Experimental models | ||
CCRF-CEM | Megrelis et al (2018) Frontiers in Immunology | Jérôme Delon |
Recombinant DNA | ||
LifeAct-mCherry | Molla-Herman A et al (2010) (Journal of Cell Science) | Dr A. Benmerah (Institut Imagine, France) |
GFP-humanCentrin1 | Azimzadeh et al (2009) (Journal of Cell Biology) | Dr A. Delouvée (Institut Curie, Paris, France) |
Centrin1-VCA-GFP and Centrin1-GFP | Obino et al (2016) (Nature Communications) | Dr P. Pierobon (Institut Cochin, Paris, France) |
TEpacVV (H187) | Klarenbeek J et al (2015) (PLoS ONE) | K. Jalink (Netherlands Cancer Institute) |
ExRaiAKAR2 | Addgene | Cat #161753 |
Antibodies | ||
Mouse anti-pericentrin | Abcam | Cat #28144 |
Rabbit anti-pericentrin | Abcam | Cat #4448 |
Rabbit anti-Arp2 | Abcam | Cat #47654 |
Mouse anti-AKAP450 | Becton Dickinson | Cat #611518 |
Mouse anti-CXCR4 human | R&D Systems | Cat #MAB172 |
Mouse anti-α tubulin | Sigma | Cat #T9026 |
Mouse anti-PKA Cα | Becton Dickinson | Cat #610981 |
AlexaFluor568 donkey anti-mouse | Life Technologies | Cat #A10037 |
AlexaFluor647 donkey anti-rabbit | Life Technologies | Cat #A32787 |
AlexaFluor plus 647 donkey anti-mouse | Life Technologies | Cat #A31573 |
Chemicals, enzymes, and other reagents | ||
RPMI 1640 GlutaMax | Gibco | Cat #61870010 |
Fetal Calf Serum (FCS) | GIBCO | Cat #A5256801 |
Penicillin and streptomycin | Eurobio | Cat #CABPES01-OU |
Amaxa Nucleofactor | Lonza | Cat #VCA-1003 |
Human AB serum | Biowest | Cat #S4190–100 |
Pan T cell Isolation Kit | Miltenyi | Cat #130-096-535 |
recombinant human SDF1-α | Peprotech | Cat #300-28 A |
VCAM-1 (CD106 Fc chimera protein) | R&D Systems | Cat #862-VC-100 |
Fura-2/AM | Molecular Probes | Cat #F1225 |
BAPTA-AM | Thermo Fisher | Cat #B6769 |
Hoechst 33342 | Molecular Probes | Cat #H1399 |
AlexaFluor488 conjugated phalloidin | Life Technologies | Cat #A12379 |
AlexaFluor568 conjugated phalloidin | Life Technologies | Cat #A12380 |
SiRActin | TebuBio | Cat #SC001 |
H89 | Calbiochem | Cat #371963 |
Rp-8-CPT-cAMPS | Biolog | Cat #C011 |
Ht31 (RRRRRRRRRLIEEAASRIVDAVIEQV) | ProteoGenix | |
Ht31-P (RRRRRRRRRLIPEAASRPVDAVIEQV) | ProteoGenix | |
Fluorsave | Millipore | Cat #345789 |
Transwell | Corning | Cat #3421 |
Software | ||
Metafluor | Meta Imaging (Roper Scientific) | |
Metamorph | Meta Imaging (Roper Scientific) | |
ImageJ / Fiji | https://imagej.net/ | |
Jamovi software | https://www.jamovi.org/ | |
Prism | GraphPad |