Figure 2.

miR-199a-5p directly regulates GPR89A in sorafenib-resistant HCC cells. (A) TargetScan database predicted that miR-199a-5p can bind to the promoter region of GPR89A. (B) Luciferase reporter assay was performed to assess the interaction between miR-199a-5p and the GPR89A promoter in sorafenib-resistant cells. (C) qRT–PCR analysis demonstrated that miR-199a-5p inhibited the expression of GPR89A. All experiments were conducted in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001; unpaired t-test.

HCC: hepatocellular carcinoma; qRT–PCR: quantitative reverse transcription–polymerase chain reaction.