FIG. 6.

RNase protection analysis of total RNA (20 μg) isolated from keratinocytes containing HPV31-wt (WT; LKP cell line [15]), HPV31-BS2 (BS2), or HPV31-BS3 (BS3) grown in monolayer cultures (M) or differentiated in raft cultures (R). Lane P contains undigested antisense probe which was transcribed from pRPA31L1. Lane S contains ³²P-end-labeled 1-kb ladder (Gibco BRL), and the sizes are indicated in nucleotides to the right. The structure of the antisense RNA probe is shown below. Parts of the L2 and L1 open reading frames are indicated. The splice acceptor site at nt 5552 is depicted by a dashed line. Spliced L1 and unspliced L2/L1 transcripts are indicated by arrows on the left of the autoradiogram.