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Kinetic parameters of WT KGA and GAC and the S482C and H461L mutants.
| Enzyme | kcat (s−1) | K0.5 glutamine (mM) | Hill slope | EC50 phosphate (mM) | IC50 glutamate (mM) |
|---|---|---|---|---|---|
| WT KGA | 7.0 ± 0.5 | 3.8 ± 0.4 | 1.2 ± 0.1 | 30 ± 4 | 4.1 ± 0.5 |
| S482C KGA | 6.6 ± 0.5 | 10.1 ± 0.7 | 1.9 ± 0.1 | - | - |
| H461L KGA | 3.9 ± 0.8 | 1.7 ± 0.3 | 1.1 ± 0.2 | - | - |
| WT GAC | 16 ± 1 | 2.9 ± 0.3 | 1.1 ± 0.1 | 16 ± 2 | 5.3 ± 0.7 |
| S482C GAC | 13.1 ± 0.9 | 5.1 ± 0.3 | 1.9 ± 0.1 | - | - |
| H461L GAC | 13.4 ± 0.8 | 2.0 ± 0.2 | 1.1 ± 0.1 | - | - |
Data are best-fit values ± SEM
