Figure 3. Preservation of active properties during retrieval is associated with recovery of synaptic proteins.

(A) Action potential firing profiles illustrating differences in retrieval quality: neuron #4 retained stable spiking, neuron #7 showed reduced amplitude consistent with leak, and neuron #6 produced only a single spike following partial aspiration. (B) UpSet plot quantifying overlap of synaptic biological process (BP) terms (annotated by SynGO) across each neuron. (C) Heatmap of top BP terms, sorted by enrichment significance. Color intensity reflects statistical significance (−log₁₀ adjusted Q-value; Q-value < 0.05 cutoff). Neuron #4 exhibited the greatest diversity of enriched terms, while neuron #6 lacked significant enrichment for synaptic signaling, despite many protein identifications.