FIG. 6.

Mutations that specifically affect the RFX1-binding site abrogate reversal of E1A 243R-induced PCNA expression by p107. Wild-type (WT) and mutant (−44/−40, −46/−39, −53GT, −56GA CAT) PCNA-CAT reporter constructs (10 μg) were cotransfected with either 0.5 μg of pCMV12S.FS (control) or pCMV12S (E1A 243R) and with or without pCMV107 expression plasmid (2 μg). The fold increase ± standard deviation in CAT expression was normalized for β-galactosidase activity and represents the average of three independent experiments done in duplicate.