Table 2.

Colloidal properties of chitosan nanoparticles formulations loaded with different concentrations of ethanolic extract of C. comosum (CE/CsNPs) and chitosan nanoparticles (CsNPs); showing their measured particle size, polydispersity Index (PDI), Zeta potential (ZP), entrapment efficiency % (EE) and loading capacity (%) data (n = 3)

Nano formulation	Concentration of the Extract (mg/100 mL)	Particle size (nm)	PDI	ZP (mV)	EE%	LC%
CsNPs	–	42.54 ± 3.65	0.29 ± 0.03	69.2 ± 2.37	–	–
CE/CsNPs-1	200	60.57 ± 3.25	0.24 ± 0.01	40.1 ± 2.56	60.84 ± 2.97	17.58 ± 1.95
CE/CsNPs-2	350	71.52 ± 4.57	0.35 ± 0.04	37.7 ± 1.91	70.15 ± 1.58	20.47 ± 2.14
CE/CsNPs-3	400	130.5 ± 1.02	0.63 ± 0.12	24.5 ± 2.45	55.75 ± 3.12	15.78 ± 1.48