Table 1. Process parameters in shake flask and bioreactor cultivations during the autotrophic production phase. Unless indicated in parentheses, final titers of Day 8 of the cultivation are given. Dissolved oxygen concentration is not controlled during shake flask cultivations. MC: multicopy.

Strain	Cultivation	Parameters				Final titer (g L−1)	Final DCW (g L−1)	Specific productivity, qp (mg g DCW−1 h−1)	Growth rate, μ (h−1)	Product yield, YaP/X or YbP/X (g g DCW−1)
		Temperature (°C)	Dissolved oxygen (%)	CO2 feed (%)	Initial OD
cadA	Shake flask	25–28	—	10	4	0.304 ± 0.016 (Day 7)	3.03 ± 0.10	1.14 ± 0.030	0.007 ± 0.0001	0.154 ± 0.002
cadA + mttA	Shake flask	25–28	—	10	4	0.628 ± 0.001 (Day 7)	2.31 ± 0.02	3.75 ± 0.000	0.006 ± 0.0000	0.480 ± 0.004
cadA + mttA	Shake flask	25–28	—	10	10	1.433 ± 0.019 (Day 7)	4.39 ± 0.00	2.96 ± 0.022	0.004 ± 0.0001	0.735 ± 0.026
cadA + mttA	Shake flask	25–28	—	10	20	1.944 ± 0.052 (Day 7)	6.33 ± 0.16	2.45 ± 0.033	0.002 ± 0.0002	1.106 ± 0.075
cadA + mttA	Shake flask	25–28	—	5	4	0.766 ± 0.039	2.57 ± 0.16	2.55 ± 0.098	0.006 ± 0.0001	0.458 ± 0.008
cadA + mttA + mfsAGAP	Shake flask	25–28	—	5	4	0.913 ± 0.026	2.48 ± 0.09	3.07 ± 0.108	0.005 ± 0.0003	0.577 ± 0.048
cadA + mttA + mfsAFDH1	Shake flask	25–28	—	5	4	0.985 ± 0.062	2.39 ± 0.16	3.42 ± 0.197	0.005 ± 0.0005	0.662 ± 0.086
cadA + mttA + mfsAGAP	Shake flask	25	—	5	4	1.111 (1.571, Day 11)	3.65 (3.78, Day 11)	3.09	0.008	0.376
MC-CBB_cadA + mttA + mfsAGAP	Shake flask	25	—	5	4	1.369 ± 0.020 (2.021 ± 0.026, Day 11)	3.91 ± 0.07 (4.14 ± 0.10, Day 11)	4.15 ± 0.061	0.009 ± 0.0003–0.008	0.463 ± 0.007
MC-CBB-IA_ cadA + mttA + mfsAGAP	Shake flask	25	—	5	4	1.908 ± 0.303	3.49 ± 0.32	5.11 ± 0.795	0.008 ± 0.0006	0.657 ± 0.088
cadA + mttA	Bioreactor, YNB + glycerol batch	30	4	5	20	0.649	4.00	0.51	No growth	0.137
cadA + mttA	Bioreactor, YNB + glycerol batch	30	8	5	20	0.710	4.07	0.5	No growth	0.151
cadA + mttA	Bioreactor, YNB + glycerol batch	30	16	5	20	0.908	4.27	0.70	No growth	0.185
cadA + mttA	Bioreactor, YNB + glycerol batch	30	16	10	20	0.648	2.91	0.83	No growth	0.157
cadA + mttA	Bioreactor, YNB + glycerol batch	25	16	10	20	1.389	4.42	1.32	0.0004	0.251
cadA + mttA + mfsAGAP	Bioreactor, YNB + glycerol batch	25	16	10	20	1.078	5.32	1.59	0.0007	0.566
cadA + mttA + mfsAGAP	Bioreactor, YNB + glycerol batch	30	16	10	20	2.692	2.91	0.90	No growth	0.321
MC-CBB-IA_ cadA + mttA + mfsAGAP	Bioreactor, YNB + glycerol batch	25	16	10	20	6.738	8.44	3.22	0.002	1.738
MC-CBB-IA_ cadA + mttA + mfsAGAP	Bioreactor, YPG batch	25	16	10	20	11.836 ± 0.262	15.73 ± 0.64	3.94 ± 0.006	0.004 ± 0.0002	0.997 ± 0.037
MC-CBB_cadA + mttA + mfsAGAP	Bioreactor, YPG batch	25	16	10	20	5.141 ± 0.057	13.22 ± 0.64	1.93 ± 0.072	0.003 ± 0.0001	0.563 ± 0.040