FIG. 3.

Quantitative measurement of productive-cycle transcripts at 33 h p.i. Autoradiographs of probed Southern blots of QRPCR products separated on polyacrylamide gels are shown. Synthetic transcript mixes (left) or individual ganglion RNAs (right) were reacted with (+) and without (−) reverse transcriptase (RT), and results are displayed in adjacent lanes. Standard, number of specific transcript molecules in serial dilution (indicated above the lines) or water blank (B) mixed with 5 μg of mouse brain RNA. Sample lanes: mock, individual ganglia from animals inoculated with medium not containing virus; TK⁻, two or three individual ganglia from animals inoculated with the tk deletion mutant dlsactk; wt, two or three individual ganglia from animals inoculated with wt HSV strain KOS; M, molecular weight marker (φX174 digested with HinfI and end labeled with ³²P). (A) The ICP4 RNA 101-nt QRPCR product, separated on a 12% polyacrylamide gel, was detected by probing with oligonucleotide 4-3. (B) The tk RNA 60-nt QRPCR product, separated on a 10% polyacrylamide gel, was detected by probing with oligonucleotide tk-3. (C) The gC RNA 109-nt QRPCR product, separated on a 10% polyacrylamide gel, was detected by probing with oligonucleotide gC-3. (D) The gH-L 81-nt QRPCR product, separated on a 12% polyacrylamide gel, was detected by probing with oligonucleotide gH-3. (E) The gH-S 44-nt QRPCR product, separated on a 12% polyacrylamide gel, was detected by probing with oligonucleotide gH-3.