Skip to main content
. 1998 Feb;72(2):1186–1194. doi: 10.1128/jvi.72.2.1186-1194.1998

FIG. 5.

FIG. 5

Strategy for mapping recombinant proviruses in cell clones. (A) Generic structure of a recombinant provirus. Above the recombinant, restriction enzyme markers are listed, and a BamHI site contained in both WH221 and WH204 is indicated beneath the recombinant. Primer sets for differential PCR analysis and the expected sizes of the amplification products are shown below the recombinant. Arrows indicate primer directions. (B) Representative differential PCR analysis of recombinant cell clones AQ2 and W12 with primer sets 1A and 1B. L; 1-kb ladder. (C) Restriction enzyme digestion analysis of AQ2 DNA amplified with primer set 1A. (D) Restriction enzyme digestion analysis of W12 DNA amplified with primer set 1B. U, undigested DNA. The 5′ structure of each recombinant proviral DNA derived from the analysis is illustrated below the restriction enzyme mapping gel in panels C and D. Symbols and abbreviations are the same as in Fig. 1 and 2.