Table 1.
O-glycan size and number, composition, and factors for compensation of orcinol and glycan-on-membrane assay
| Species | Tissue | Size: Average (SEM) number of monosaccharides | Average (SEM) Number of non-amine hexoses |
Average (SEM) number of terminal cis-hydroxy groups | Multiplication factor for orcinol assay compensated with PGM | Multiplication factor for glycan-on-membrane assay compensated with PGM |
|---|---|---|---|---|---|---|
| Atlantic salmon | Gill | 3.4 (0.095) | 0.780 (0.088) | 1.49 (0.041) | 2.4 | 0.86 |
| Skin | 2.4 (0.029) | 0.249 (0.015) | 1.10 (0.0089) | 7.6 | 1.2 | |
| Barramundi | Gill | 6.0 (0.47) | 0.563 (0.054) | 1.53 (0.076) | 3.4 | 0.83 |
| Skin | 5.5 (0.59) | 0.375 (0.063) | 1.40 (0.086) | 5.1 | 0.91 | |
| Oral | 5.1 (0.83) | 0.282 (0.047) | 1.27 (0.085) | 6.7 | 1.0 | |
| Intestine | 5.5 (0.11) | 1.24 (0.11) | 1.76 (0.072) | 1.5 | 0.73 | |
| PGM | 4.2 | 1.90 | 1.28 |