FIG. 3.
Development of the RT-PCR assay with a 5′-GC-clamped T20 primer and a 717-nt vRNA template. Lane 1, DNA markers; lane 2, complete reaction, showing a broad poly(A)+ band (see Materials and Methods); lanes 3 through 6, reactions without (−) template, RNP, Moloney murine leukemia virus RT, and 5′-GC-clamped T20 primer, respectively. WT 717 nt, wild-type influenza virus-like 717-nt vRNA.