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. 1998 Feb;72(2):1280–1286. doi: 10.1128/jvi.72.2.1280-1286.1998

FIG. 3.

FIG. 3

Development of the RT-PCR assay with a 5′-GC-clamped T20 primer and a 717-nt vRNA template. Lane 1, DNA markers; lane 2, complete reaction, showing a broad poly(A)+ band (see Materials and Methods); lanes 3 through 6, reactions without (−) template, RNP, Moloney murine leukemia virus RT, and 5′-GC-clamped T20 primer, respectively. WT 717 nt, wild-type influenza virus-like 717-nt vRNA.

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