FIG. 3.

Western blot analysis of 15-kDa protein synthesis in VVindA14L-infected cells. BSC40 cells were infected (5 PFU/cell) with WR (lanes 1 to 4) or with VVindA14L in the presence (lanes 5 to 8) or absence (lanes 9 to 12) of IPTG (2 mM). Cells were harvested at different times postinfection as indicated and lysed in 1× sample buffer. Proteins were fractionated by SDS-PAGE (12% polyacrylamide gel) and transferred to a nitrocellulose membrane. The membrane was cut in two pieces; the upper part containing the higher-molecular-mass proteins was reacted with anti-39-kDa-protein antibodies (A), and the lower portion of the membrane was incubated with anti-15-kDa-protein serum (B).