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. 1998 Feb;72(2):1287–1296. doi: 10.1128/jvi.72.2.1287-1296.1998

FIG. 6.

FIG. 6

Viral structures distinguished in the cytoplasm of cells after a 24-h infection with WR VV, VVindA17L in the absence of IPTG, or VVindA14L in the presence or absence of IPTG. (A) IVs accumulate in cells infected with WR VV. Arrows point to condensed DNA inside the IVs. (B) Electron-dense masses (asterisk) surrounded by tubular (T) and vesicular (V) membranous elements are assembled in cells infected with VVindA17L in the absence of IPTG (when the 21-kDa protein is not expressed). (C) Cells infected with the VVindA14L virus in the presence of IPTG exhibit characteristic foci of viroplasmic matrix (F) surrounded by viral crescents (c), IVs, and membranous elements (arrows). (D) In the absence of IPTG (when the 15-kDa protein is not present), the VVindA14L virus induces the formation of dense structures similar to viral factories (asterisks) and crescent-like structures (arrows) that do not attach to the surfaces of the masses. IV-like virions (arrowhead) of irregular shape are occasionally seen. Bar, 300 nm.