TABLE 1.
Yeast strains used
| Straina (reference) | Plasmid | Plasmid description |
|---|---|---|
| YHL1282 (28) | pHL449-1 | Wild-type Tf1-neoAI |
| YHL1554 (28) | pHL476-3 | Tf1-neoAI with IN frameshift |
| YHL1836 (1) | pHL490-80 | Tf1-neoAI with PR frameshift |
| YHL1887 | pHL449-1b | Tf1 expression plasmid that contained mutation 1 (A1311T) |
| YHL1888 | pHL449-1b | Tf1 expression plasmid that contained mutation 2 (P816S) |
| YHL1890 | pHL449-1b | Tf1 expression plasmid that contained mutation 3 (S1009L) |
| YHL1891 | pHL449-1b | Tf1 expression plasmid that contained mutation 4 (L929F) |
| YHL1895 | pHL449-1b | Tf1 expression plasmid that contained mutation 5 (S749L) |
| YHL1917 | pHL449-1b | Tf1 expression plasmid that contained mutation 9 (P903L) |
| YHL1826 | pHL449-1b | Tf1 expression plasmid that contained mutation 10 (E1142K) |
| YHL2053 | pHL495 | Tf1-neoAI plasmid with mutation 1 isolated from YHL1887 |
| YHL2057 | pHL496 | Tf1-neoAI plasmid with mutation 2 isolated from YHL1888 |
| YHL2061 | pHL498 | Tf1-neoAI plasmid with mutation 3 isolated from YHL1890 |
| YHL2065 | pHL499 | Tf1-neoAI plasmid with mutation 4 isolated from YHL1891 |
| YHL2069 | pHL502 | Tf1-neoAI plasmid with mutation 5 isolated from YHL1895 |
| YHL2085 | pHL519 | Tf1-neoAI plasmid with mutation 9 isolated from YHL1917 |
| YHL2089 | pHL575 | Tf1-neoAI plasmid with mutation 10 isolated from YHL1826 |
| YHL2165 | pHL680-1 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL495 |
| YHL2170 | pHL681-3 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL496 |
| YHL2174 | pHL682-3 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL498 |
| YHL2178 | pHL683-2 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL499 |
| YHL2182 | pHL684-2 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL502 |
| YHL2198 | pHL688-1 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL519 |
| YHL2202 | pHL689-2 | Tf1-neoAI plasmid with BsrGI-BamHI fragment from pHL575 |
| YHL2206 | pHL715-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL495 |
| YHL2230 | pHL731-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL496 |
| YHL2210 | pHL717-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL498 |
| YHL2214 | pHL718-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL499 |
| YHL2234 | pHL732-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL502 |
| YHL2222 | pHL723-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL519 |
| YHL2226 | pHL724-1 | Tf1-neoAI plasmid with AvrII-BsrGI fragment from pHL575 |
| YHL5282 | pHL1187-2 | PCR reconstruction of mutation 2 from plasmid pHL731-1 |
| YHL5274 | pHL1183-2 | PCR reconstruction of mutation 5 from plasmid pHL732-1 |
a
All plasmids were transformed into YHL912 (h− ura4-294 leu1-32) (2).
b
Contained hydroxylamine-generated mutation.