TABLE 3.
Positions of mutations in Tf1 that affected transposition
| Mutant | Fragment with mutation | Substitution | Protein domain |
|---|---|---|---|
| 1 | BsrGI-BamHI of pHL680-1 | Ala1311→Thr | DNA binding domain of IN |
| 2 | AvrII-BsrGI of pHL731-1 | Pro816→Ser | RH domain of RT |
| 3 | BsrGI-BamHI of pHL682-3 | Ser1009→Leu | Catalytic core of IN |
| 4 | AvrII-BsrGI of pHL718-1 | Leu929→Phe | Zn finger domain of IN |
| 5 | AvrII-BsrGI of pHL732-1 | Ser749→Leu | RH domain of RT |
| 9 | AvrII-BsrGI of pHL723-1 | Pro903→Leu | Zn finger domain of IN |
| 10 | BsrGI-BamHI of pHL689-2 | Glu1142→Lys | Catalytic core of IN |