TABLE 4.
Quantitative transposition frequencies of mutants 2 and 5 and PCR reconstructions
| Strain | Plasmid description | G418r colonies/FOAr colonies | % Transposition |
|---|---|---|---|
| 1282 | Wild-type Tf1-neoAI | 78/3,796 | 2.05 |
| 1554 | Tf1-neoAI with IN frameshift | 3/5,086 | 0.06 |
| 1836 | Tf1-neoAI with PR frameshift | 0/4,290 | <0.02 |
| 2234 | Mutant 5 (S749L) | 6/5,252 | 0.11 |
| 5274 | Mutant 5; PCR reconstruction 1 | 6/3,376 | 0.18 |
| 5278 | Mutant 5; PCR reconstruction 2 | 5/3,331 | 0.15 |
| 2230 | Mutant 2 (P816S) | 7/5,745 | 0.12 |
| 5282 | Mutant 2; PCR reconstruction 1 | 7/4,707 | 0.15 |
| 5286 | Mutant 2; PCR reconstruction 2 | 5/2,322 | 0.22 |