FIG. 1.
Amplification and molecular cloning of the 3.3-kbp 3′-terminal fragment of EIAVPV. A 3.3-kbp fragment from EIAVPV-infected cell cultures was amplified by PCR, blunt ended, and digested with NcoI prior to ligation with NcoI/SmaI-digested pLG338/30 vector DNA. The resultant pLG338/PV3.3 molecular clones were digested with NcoI and EcoRI, and the 3.3-kbp EIAVPV fragment was inserted into the infectious molecular clone 19-2-6A. This produced chimeric proviral molecular clones designated EIAVPV3.3.