KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| ||
| Rabbit monoclonal anti-active caspase-3 BUV395 (clone C92–605.rMab) | BD Biosciences | Cat# 570180; RRID:AB_3685569 |
| Rat monoclonal anti-B220 APC-Cy7 (clone RA3–6B2) | BioLegend | Cat# 103224; RRID:AB_313007 |
| Mouse monoclonal anti-Bcl-2 AF647 (clone BCL/10C4) | BioLegend | Cat# 633510; RRID:AB_2274702 |
| Mouse monoclonal anti-Bcl-2 PE-Cy7 (clone BCL/10C4) | BioLegend | Cat# 633512; RRID:AB_2565247 |
| Rat monoclonal anti-CD3 PE/Dazzle™ (clone 17A2) | BioLegend | Cat# 100246; RRID:AB_2565883 |
| Rat monoclonal anti-CD4 BV510 (clone GK1.5) | BioLegend | Cat# 100449; RRID:AB_2564587 |
| Rat monoclonal anti-CD8alpha BV510 (clone 53–6.7) | BioLegend | Cat# 100752; RRID:AB_2563057 |
| Rat monoclonal anti-CD16/CD32 (clone 2.4G2) | Bio X Cell | Cat# BE0307; RRID:AB_2736987 |
| Rat monoclonal anti-CD38 BV510 (clone 90) | BD Biosciences | Cat# 740129; RRID:AB_2739886 |
| Mouse monoclonal anti-CD45.1 AF700 (clone A20) | BD Biosciences | Cat# 561235; RRID:AB_10611577 |
| Mouse monoclonal anti-CD45.1 PE (clone A20) | Thermo Fisher Scientific | Cat# 12–0453-83; RRID:AB_465676 |
| Mouse monoclonal anti-CD45.1 PerCP-Cy5.5 (clone A20) | Thermo Fisher Scientific | Cat# 45–0453-82; RRID:AB_1107003 |
| Mouse monoclonal anti-CD45.2 BV650 (clone 104) | BioLegend | Cat# 109836; RRID:AB_2563065 |
| Mouse monoclonal anti-CD45.2 PB (clone 104) | BioLegend | Cat# 109820; RRID:AB_492872 |
| Mouse monoclonal anti-CD45.2 PerCP-Cy5.5 (clone 104) | BioLegend | Cat# 109828; RRID:AB_893350 |
| Rat monoclonal anti-CD93 BUV615 (clone AA4.1) | BD Biosciences | Cat# 751080; RRID:AB_2875116 |
| Rat monoclonal anti-CD93 PE-Cy7 (clone AA4.1) | BioLegend | Cat# 136506; RRID:AB_2044012 |
| Hamster monoclonal anti-CD95 PE-Cy7 (clone JO2) | BD Biosciences | Cat# 557653; RRID:AB_396768 |
| Rat monoclonal anti-CD138 (clone 281–2) | BD Biosciences | Cat# 553712; RRID:AB_394998 |
| Rat monoclonal anti-CD138 APC (clone 281–2) | BioLegend | Cat# 142506; RRID:AB_10962911 |
| Rat monoclonal anti-CD138 BV785 (clone 281–2) | BioLegend | Cat# 142534; RRID:AB_2814047 |
| Rat monoclonal anti-CD138 PE (clone 281–2) | BioLegend | Cat# 142504; RRID:AB_10916119 |
| Rat monoclonal anti-CD138 PerCP-Cy5.5 (clone 281–2) | BioLegend | Cat# 142510; RRID:AB_2561601 |
| Armenian hamster monoclonal anti-CD183 PE-Cy7 (clone CXCR3–173) | Thermo Fisher Scientific | Cat# 25–1831-82; RRID:AB_2573416 |
| Rat monoclonal anti-CD184 BUV805 (clone 2B11/CXCR4) | BD Biosciences | Cat# 741979; RRID:AB_2871282 |
| Rat monoclonal anti-CD184 BV421 (clone 2B11/CXCR4) | BD Biosciences | Cat# 562738; RRID:AB_2737757 |
| Rat monoclonal anti-CD267 BV421 (clone 8F10) | BD Biosciences | Cat# 742840; RRID:AB_2741091 |
| Rat monoclonal anti-CD326 BV711 (clone G8.8) | BioLegend | Cat# 118233; RRID:AB_2632775 |
| Rat monoclonal anti-GL7 AF647 (clone GL7) | BioLegend | Cat# 144606; RRID:AB_2562185 |
| Rat monoclonal anti-I-A/I-E AF700 (clone M5/114.15.2) | BioLegend | Cat# 107622; RRID:AB_493727 |
| Rat monoclonal anti-I-A/I-E PE (clone M5/114.15.2) | BioLegend | Cat# 107608; RRID:AB_313323 |
| Rat monoclonal anti-IgA APC (clone mA-6E1) | Thermo Fisher Scientific | Cat# 17–4204-80; RRID:AB_2848293 |
| Rat monoclonal anti-IgD FITC (clone 11–26c.2a) | BD Biosciences | Cat# 553439; RRID:AB_394859 |
| Rat monoclonal IgG2a kappa isotype control (clone RTK2758) | BioLegend | Cat# 400544; RRID:AB_11147167 |
| Goat polyclonal anti-IgG AF647 | Jackson ImmunoResearch Labs | Cat# 115–605-164; RRID:AB_2338913 |
| Human IgG1 isotype control | Bio X Cell | Cat# BE0297; RRID:AB_2687817 |
| Donkey polyclonal anti-IgM DyLight 405 | Jackson ImmunoResearch Labs | Cat# 715–475-140; RRID:AB_2340838 |
| Mouse monoclonal anti-IκBα APC (clone L35A5) | Cell Signaling Technology | Cat# 46001; RRID:AB_3697353 |
| Rat monoclonal anti-Ly-6A/E AF700 (clone D7) | BioLegend | Cat# 108142; RRID:AB_2565959) |
| Rabbit monoclonal anti-Mcl-1 AF647 (clone D2W9E) | Cell Signaling Technology | Cat# 78471; RRID:AB_2799914 |
| Rabbit monoclonal anti-Mcl-1 PB (clone D2W9E) | Cell Signaling Technology | Cat# 85415; RRID: AB_3697355 |
| Mouse monoclonal anti-TIGIT BV421 (clone 1G9) | BioLegend | Cat# 142111; RRID:AB_2687311 |
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| Chemicals, peptides, and recombinant proteins | ||
|
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| APRIL (mouse) (multimeric) (rec.) | Adipogen Life Sciences | Cat# AG-40B-0089 |
| BAFF-R (human):Fc (human) (rec.) | Adipogen Life Sciences | Cat# AG-40B-0027 |
| Bovine Fibronectin | Sigma-Aldrich | Cat# F1141 |
| Bovine Serum Albumin, Fraction V (Modified Cohn) | Sigma-Aldrich | Cat# 12660 |
| Corn oil | Sigma-Aldrich | Cat# C8267 |
| EDTA (0.5 M), pH 8.0, RNase-free | Thermo Fisher Scientific | Cat# AM9261 |
| 16% Formaldehyde, Methanol-Free | Cell Signaling Technology | Cat# 126060S |
| Gibco™ ACK Lysing Buffer | Thermo Fisher Scientific | Cat# A1049201 |
| Gibco™ Fetal Bovine Serum | Thermo Fisher Scientific | Cat# A3160601 |
| Gibco™ HBSS (10×), calcium, magnesium, no phenol red | Thermo Fisher Scientific | Cat# 14065056 |
| Gibco™ Penicillin-Streptomycin-Glutamine (100×) | Thermo Fischer Scientific | Cat# 10378016 |
| HEPES | Sigma-Aldrich | Cat #H3375 |
| Imject Alum Adjuvant | Thermo Fisher Scientific | Cat# 77161 |
| Isoflurane Inhalation Solution | Henry Schein | Cat# 1182097 |
| Lactated Ringer’s Solution | Henry Schein | Cat# 14792 |
| Lipopolysaccharides from Escherichia coli O111:B4 | Sigma-Aldrich | Cat# L-2630 |
| 2-Mercaptoethanol (50 mM) | Thermo Fisher Scientific | Cat# 31350010 |
| 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl)Amino)-2-Deoxyglucose) | Thermo Fisher Scientific | Cat# N13195 |
| NP(32)-KLH | Biosearch Technologies | Cat# N-5060–5 |
| PBS, 10X Powder, pH 7.4 | Thermo Fisher Scientific | Cat# BP665–1 |
| Qtracker™ 705 Vascular Labels | Thermo Fisher Scientific | Cat# Q21061MP |
| Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein | R&D Systems™ | Cat# 796IC050 |
| Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein | R&D Systems™ | Cat # 643VM050 |
| RPMI 1640 Media, No Glutamine | Cytiva | Cat# SH30096.01 |
| Sulfatrim Pediatric Suspension (sulfamethoxazole and trimethoprim oral suspension USP) | PAI Pharmaceutical Associates, Inc. | NDC# 00121–0854-16 |
| SynstatinVLA-4 custom peptide (CD138 core protein residues 210–236) | GenScript | N/A |
| TACI (human):Fc (human) (rec.) | Adipogen Life Sciences | Cat# AG-40B-0079 |
| Tamoxifen | Sigma-Aldrich | Cat# T5648 |
| Triton™ X-100 Surfact-Amps™ Detergent Solution | Thermo Fisher Scientific | Cat# 28314 |
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| Critical commercial assays | ||
|
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| AccuCount Fluorescent Particles | Spherotech | Cat# ACFP705 |
| BD Cytofix/Cytoperm ™ Fixation/Permeabilization Solution Kit | BD Biosciences | Cat# 554714 |
| BD Horizon ™ Brilliant Stain Buffer | BD Biosciences | Cat# 566349 |
| CD43 (Ly-48) MicroBeads, mouse | Miltenyi Biotec | Cat# 130–049-801 |
| Nunc ™ Lab-Tek ™ Chambered Coverglass | Thermo Fisher Scientific | Cat # 155411PK |
| UltraComp eBeads ™ Compensation Beads | Thermo Fisher Scientific | Cat# 01222242 |
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| Experimental models: Organisms/strains | ||
|
| ||
| Mouse: Blimp1-ERT2-Cre-TdTomato (BEC) | Jing et al., 2024. | N/A |
| Mouse: Blimp1-YFP: B6.Cg-Tg(Prdm1-EYFP)1Mnz/J | The Jackson Laboratory | JAX Stock# 008828; RRID:IMSR_JAX:008828 |
| Mouse: B1–8hi: CBy.129P2(B6)-Ightm1Mnz/J | The Jackson Laboratory | JAX Stock# 007775; RRID:IMSR_JAX:007775 |
| Mouse: B6: C57BL/6J | The Jackson Laboratory | JAX Stock# 000664; RRID:IMSR_JAX:000664 |
| Mouse: B6.C-Tg(CMV-cre)1Cgn/J | The Jackson Laboratory | JAX Stock# 006054; RRID:IMSR_JAX:006054 |
| Mouse: CD45.1: B6-Ly5.1/Cr | Charles River Laboratories | NCI Stock# 564 |
| Mouse: CD138 KO: Sdc-1−/− | Alexander et al.46 | N/A |
| Mouse: Rosa26LSLTomato: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | JAX Stock# 007914; RRID:IMSR_JAX:007914 |
| Mouse: Rosa26LSLYFP: B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | The Jackson Laboratory | JAX Stock# 006148; RRID:IMSR_JAX:006148 |
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| Software and algorithms | ||
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| Adobe After Effects | Adobe | N/A |
| Excel | Microsoft | RRID:SCR_016137 |
| FlowJo | FlowJo, LLC | RRID:SCR_008520 |
| Imaris | Bitplane | RRID:SCR_007370 |
| MATLAB | MathWorks | RRID:SCR_001622 |
| Olympus Fluoview FV10-ASW | Olympus | RRID:SCR_014215 |
| Prism | Graphpad | RRID:SCR_002798 |