FIG. 6.
Specific lysis observed on target cells sensitized with different peptides: the original peptide Nef 128-137/136A (○), the selected peptide Nef 128-137/136T (▵), and the mutant peptide Nef 128-137/131E+136T (□). Target cells were autologous B-LCLs preincubated for 1 h with 10-fold serial dilutions of the different peptides. The CTL cell lines were obtained by stimulation of PBMC with the original Nef 128-137/136A peptide from macaque C16 (a) and macaque C18 (b). The E/T cell ratio was 10:1.