TABLE 3.
Formation of PRV(Kaplan) infectious centers and plaques on susceptible BHK(TK−) cells, resistant 95-19 cells, and HVEM-expressing 95-19 cells
| Test cells | Input PFUa | Infectious centersb | Test cell plaquesc |
|---|---|---|---|
| BHK(TK−) | 5 × 103 | 39 | 50 |
| 5 × 102 | 4 | 10 | |
| 5 × 101 | 1 | 0 | |
| 95-19/pcDNA3 | 5 × 106 | TNTCd | TNTC |
| 5 × 105 | 39 | 51 | |
| 5 × 104 | 4 | 4 | |
| 95-19/HVEM | 5 × 106 | TNTC | TNTC |
| 5 × 105 | 27 | 31 | |
| 5 × 104 | 1 | 4 | |
| 5 × 103 | 0 | 0 |
a
Determined by titration of virus stock on Vero cells.
b
Number of plaques obtained following seeding of infected test cells onto a Vero cell monolayer.
c
Number of plaques observed on the infected test cell monolayer.
d
TNTC, too numerous to count.