TABLE 2.
Radioactivity in nucleocapsids and virions 24 h posttransfectiona
| RNA | Radioactivity relative to that in SIN
|
|
|---|---|---|
| Nucleocapsids | Virions | |
| Expt 1 | ||
| SIN Toto54 | 1 | 1 |
| SIN(E2:D248Y) | 1.2 | 1.0 |
| SIN(RRE1) | 0.6 | <0.001 |
| SIN(RRE1)(E2:D248Y/E1:Q411L) | 0.9 | <0.001 |
| RR64 | 0.06 | 0.16 |
| Expt 2 | ||
| SIN Toto54 | 1 | 1 |
| SIN(E2:D248Y) | 1.3 | 0.6 |
| SIN(RRE1) | 0.9 | <0.002 |
| SIN(RRE1)(E2:D248Y/E1:Q411L) | 1.3 | <0.002 |
a
BHK cells were transfected with RNA and labeled with [3H]uridine from 8 to 24 h, and radiolabel in intracellular nucleocapsids and in released virions at 24 h was assayed by sucrose gradient sedimentation.