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Binding of amphotropic and 10A1 pseudotyped MLV to fibroblasts and hematopoietic cell lines. Cells were incubated with virus supernatants. Total virions, as well as the viral glycoprotein gp70SU, bound to the cell surface were then stained with an anti-gp70 antibody, followed by binding of a biotinylated secondary antibody and streptavidin-phycoerythrin. The cells were then analyzed on a flow cytometer.
