FIG. 2.

Western blot analysis of proteins synthesized in 293 cells which had been infected with 10 PFU of RSV per cell (lane 1) or infected with the vaccinia virus recombinant vTF7-3 (lanes 2 to 6) and mock transfected (lane 2) and then transfected with 200 ng of N plasmid and 100 ng of P plasmid (lane 3) or the same amounts of N and P plasmids supplemented with 30 ng (lane 4), 60 ng (lane 5), or 100 ng (lane 6) of NS1 plasmid. Cells were harvested 18 h (lane 1) or 40 h (lanes 2 to 6) postinfection, and lysates were electrophoresed on a 4 to 20% gradient gel and transferred to nitrocellulose. The upper blot was probed with a polyclonal antiserum against gradient-purified, disrupted RSV virions (17), which is reactive with most of the RSV structural proteins including G, N, P, M, and SH. The lower blot was probed with an antibody against a C-terminal peptide of the NS2 protein, which recognizes both the NS1 and NS2 proteins in RSV-infected cells (lane 1) and NS1 in the plasmid-transfected cells (lanes 4 to 6).